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Benincasa hispida – "Brihatphala" - A Review

Benincasa hispida "Brihatphala" - A Review


Benincasa hispida "Brihatphala" - A Review

Introduction:

The genus name was given to it by famous Italian botanist, Gaetano Savi,in 1818 to honour Giuseppe Benincasa, an Italian patron of botany. He called the plant as Benincasa cerifera " wax bearing". Hispida means " rough hairy"as whole parts of plant contains rough hair.13

Vernacular names:

Synonyms : Benincasa cerifera, Cucurbita hispida

Sanskrit : Kushmanda,Brihatphala, Karkotika, Kumbhaphal, Somayashtika

English : Ash gourd, winter melon, green pumpkin, wax gourd, white gourd

Marathi : Kohla

Hindi : Petha, Raksa

Kannada : Budhikumbala

Malayalam : Kum palam

Tamil : Pusanikka

Telgu : Budidajummea

Tulu : Karkumbuda

Gujarati : Kolu

Assamese : Komara

Nepal : Kubindo

French : Courgette velue

German : Wintermelone

Japanese : Togan

Korean : Donggwa

Arab : Muhadba

Malaysia : Kumbalam

In Nature :

Plant probably a native of Malaysia, but is now found throughout tropics. It is cultivated for its fruits throughout the plain of India, Burma and Ceylan, and on hills upto 4000ft. It is very well known vegetable in India, China, Philippines, Thailand, Vietnam and elsewhere in Asia14. In plains seeds are sown during Feb- Mar and on hills in Mar- May.

Propagation and cultivation :

Varieties : Co 2' is a high yielding released from Tamil Nadu. It bears small fruits weighing 3 kg.

Cultivation :

Crop can be grown in plains in late winter by adopting polyhouse technology. It involves raising the seedlings during Dec- Jan in small polythene bags. Seedlings having 3-4 true leaves should be transplanted in the pits in 1st week of Feb when danger of frost is over. About 1,150 1,350 seedlings are required for planting 0.405 hector land. Improved varieties Co 1' & Co 2' are suitable for commercial cultivation . By adopting this technique the fruits can be taken 1- 1 mth in advance than normal method of direct seed sowing in field.

The optimum temperature when growing is 73 -820F and moderate rainfall but no hot, humid tropics. It requires long growing season of 5 months to develop to maturity, though they can be harvested at different immature stages. Wax gourd are drought tolerant. Where large fruits are wanted they are grown on flat grounds otherwise they are allowed to climb on fences.

Cultivated largely in uttar Pradesh, Punjab, rajasthan, and bihar

Parts used :

Seeds, fruits and fruit juice

Macroscopic :

Ash gourd or White gourd melon is a large trailing gourd climbing by means of

tendrils 10. It sprawl over ground or climb like pumpkin.

Stems: Thick, furrowed and hairy

Leaves : large and hispid beneath and rough to touch

Flowers : yellow, unisexual with male peduncle 7.5-10cm long and female peduncle

shorter.

Fruits : In immature fruits the skin colour can be of various shades from light or dark green to purple blue depending on the variety. The flesh is white and the fruit is coverd with downy hairs. The mature one are remarkable with distinctive waxy layer. This is protective layer for which it can be stored for longer period. They vary in shape from truly spherical, to round with flattened ends or just like Chinese pillow shape' . Broadly cylindric, 30-45cm long, hairy throughout 10 weighing around 11- 45 kg and the smallest one around 2.2- 4.5 kg and 30 38 cm long

Microscopic :

Mature fruit shows cuticularised epicarp consisting of single layered, squarish or

slightly tangentially elongated cells of epidermis, outer tangential walls of epidermis

thickened and cuticularised; a few epidermal cells divide periclinically and become 2 or 3

layered; mesocarp has a heterogenous structure consisting of multilayered hypodermis

composed of tangentially elongated, thin-walled, parenchymatous cells; immediately

within this is a zone of thick-walled, multilayered, lignified sclereids with the outer one

to three layers thicker than the inner 2 to 6 or more layers; beneath this zone, thin walled

tangentially elongated, parenchymatous cells present, their size gradually

increasing from those at periphery to those inside of mesocarp, the latter becoming

circular having conspicuous intercellular spaces; vascular bundles poorly developed,

bicollateral, found scattered throughout mesocarp.

Powder - Dirty brown; shows numerous fragments of thin-walled, tangentially elongated

and circular parenchymatous cells, numerous sclereids in groups and singles and a few

fragments of xylem vessels having spiral thickenings.

IDENTITY, PURITY AND STRENGTH

Foreign matter - Not more than 1 per cent

Total Ash - Not more than 12 per cent

Acid-insoluble ash - Not more than 1 per cent

Alcohol-soluble extractive- Not less than 10 per cent

Water-soluble extractive - Not less than 24 per cent

T.L.C.

T.L.C. of alcoholic extract on Silica gel 'G' plate using Benzene: Ethylacetate

(9:1) shows under U.V. (366nm) two fluorescent zones at Rf. 0.71 and 0.79 (both violet).

On exposure to Iodine vapour eight spots appear at Rf. 0.07, 0.18, 0.28, 0.40, 0.50, 0.59,

0.71 and 0.79 (all yellow). On spraying with Vanillin-Sulphuric acid reagent and heating

the plate at 105C for ten minute six spots appear at Rf. 0.07, 0.18, 0.40, 0.50, 071 and

0.79 (all violet).

Phytochemistry :

Major compound identified are:

(E) 2- hexenal, n hexenal, n- hexyl formate is powerful green fruity, pungent odour

n- hexenal is powerful, penetrating fatty green, grassy odour and n- hexyl formate is strongly penetrating, ethered fruity odour. It contains 0.5gm glucose & 0.5 gm fructose/ 100gm but no sucrose. However, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, 2,3,5-trimethylprazine, 2-methylpyrazine, and 2-ethyl-5-methylpyrazine were the major compounds in the wax gourd beverage.

Roots - Pentacyclic triterpene, bryonolic acid

Fruits - Lupeol, - sitosterol & their acetates, cucurbitin, rhamnose, mannitol, triacontenol, alkakali,fat,vitamin,glucose, adenine, trigonelline, histedine. The waxy layer of fruit contains pentacyclic triterpene isomultiflorenol acetate. The aroma of fruit & beverage differs because former contains hexanal compound & latter pyrazine compound.

Seeds 24z ethylidene cholesterol- 7 enol (avenasterol ) & 24 - ethyl cholesterol 7,25 dienol. Cucurbita 5,24 dienol is isolated from the leaves, stems, pericarp & roots.

Triterpene alcohols, euphol, tirucallol, cycloartenol, taraxerol, & - amyrin, butyrospermol, isomultiflorenol, 24 methylene cycloartanol, multiflorenol

Pharmacological/biological activities :

Anti-Ulcer: Extracts of Benincasa hispida prevent development of experimental ulcers: Used in Ayurveda for peptic ulcers, the study showed extracts of BH may be a natural drug with anti-ulcer activity.13

Anti-angiogenic Effect: Study showed the seed extract of Bh decreased bFGF-induced endothelial cell proliferation and tube formation in a dose-dependent manner. It showed no cytotoxicity and showed potent inhibitory effect on bFGF-induced angiogenesis in vivo. Seed extract of BH supports its anti-angiogenic property through inhibition of endothelial cell proliferation.

Gastroprotective / Anti-Ulcer / Antioxidant: (1) Study results were comparable with the omeprazole treated group. Study suggest BH possess significant antiulcer and well as antioxidant property. (2) Study showed decrease in ulcer index in animals treated with fruit extract of Bh. BH has been shown to contain active principles terpenes, flavanoid C, glycosides and sterols which have antioxidant effects, probably helping inhibit gastric mucosal damage by scavenging free radicals and repressing production of superoxide dismutase.

Bronchodilator Effect: EFFECT OF METHANOLIC EXTRACT OF BENINCASA HISPIDA AGAINST HISTAMINE AND ACETYLCHOLINE INDUCED BRONCHOSPASM IN GUINEA PIGS: The ME of BH showed excellent protection against histamine-induced bronchospasm probably through an antihistamin activty (H1 receptor-antagonism).

Opioid Withdrawal Benefit: Study showed the juice of Bh showed significant activity against symptoms of morphine withdrawal. Results suggest a potential for Bh in preventing the development of morphine addiction and suppression of opioid withdrawal in animals.

Antinociceptive / Antipyretic: Study results indicate that the ethanolic extract of Benincasa hispida possesses potent antinociceptive and antipyretic effects and pharmacologically justifies its folkloric use for fever and pain conditions.

Antidiarrheal: Study showed the methanolic extract of fruit of Bh showed significant inhibitory activity against castor oil-induced diarrhea and inhibited PGE2 induced enteric pooling in rats. Results establish its efficacy as an antidiarrheal agent.

Antioxidant / Alzheimer's disease: Results revelaed chronic treatment of Bh pulp extract markedly decreased lipid peroxidation level, significantly increased superoxide dismutase, CAT and reduced glutathione level in different parts of the brain. Study showed the antioxidant property of BH maybe beneficial in the management of colchicene-induced rat model of Alzheimer's disease.

Anorectic / Potential Anti-Obesity Benefit : Study investigated the anorectic effect of the methanol extract of BH in Swis albino mice. Results reveal, for a the first time, a possible anorectic activity of BH probably through CNS mediation, with no effect on gastric emptying. Further studies are suggested for its antiobesity potential

References :

Asolkar , L.V Glossary of Indian Medicinal Plants with active principles Part , Edi 1992, 119

The wealth of India Raw Materials Vol (A-B) 1948, 173

Nadkarni , Indian Materia Medica Vol , 186

C.P Khare Encyclopedia of Indian Medicinal Plants edi 2007, 97

Sheth.Ashok.K Herbs of Ayurveda Vol , 2005

V.S. Agarwal Drug plants of India , 1997, 217

John Lindley & Thomas Moore The encyclopedia/treasury of botany of vegetable kingdom A glossary of botanical terms 1995

Kirtikar basu Indian medicinal plants - Vol 1994

G.A Stuart MD Chinese material medica vegetable kingdom - 1911

P. P. Joy J. Thomas Samuel Mathew Baby P. Skaria Medicinal Plants 1998, 130

Ayurvedic Pharmacopia of India Part , Vol V, 1st edi, 1st Jan-2004

Chopra's Indigeneous Drug's of India

Heiser.B.Charles The Gourd book 1920, 61 Jules Janick, Robert E. Paull The encyclopedia of fruits and nuts ,276

Kumar A, Rama I.T Antiulcer properties of methalonic extract of benincasa hispida (Thumb) Cogn.

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